For informational and research purposes only.
For informational and research purposes only.
A step-by-step guide to mixing your peptide and measuring each dose.
One question at a time, with every number explained.
Step 1 of 7 · Getting started
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Everything the calculator does comes down to these three numbers.
How much peptide is in the whole vial, printed on the label in mg (milligrams) or mcg (micrograms). It doesn’t change when you add water.
1 mg = 1,000 mcg
The amount of liquid you mix into the vial, in mL (milliliters). It spreads the peptide out; it doesn’t add any.
Measured in mL. It isn’t the size of the vial.
How much peptide is in each mL of the mixed liquid. More water makes it weaker, so each dose is a larger amount of liquid.
Amount in the vial ÷ water added = strength. For example, 10 mg ÷ 2 mL = 5 mg/mL.
Direct answers about the math, the units, and the limits of a calculator.
Reconstitution means dissolving powder in a suitable liquid. The total amount and the liquid volume after mixing tell you how much is in each mL. Follow your product’s instructions for the liquid, volume, and preparation.
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For an educational calculation, first check a verified Certificate of Analysis (COA) for the same product and batch. If it reports a measured amount of the intended compound per vial, use that amount. A purity percentage alone is not a fill amount. Without a matching measured result, identify the label as an estimate. A batch result does not measure every vial. For a prescribed medication, ask your pharmacist to resolve any difference from the label before changing the calculation.
The calculation depends on how much peptide is in the vial. Use a matching lab report with an amount per vial if you have one; otherwise, use the label as an estimate. More peptide in the vial means more peptide in each mL. A number such as 10.83 mg can be entered exactly, but extra decimal places do not prove the amount is correct.
Divide the total amount in the vial by the liquid volume after mixing. For example, 5 mg in 2 mL gives 2.5 mg in each mL, written as 2.5 mg/mL. That is also 2,500 mcg/mL. The calculator shows the concentration in mg/mL even if you entered mcg.
1 mg = 1,000 mcg. Divide mcg by 1,000 to get mg: 250 mcg = 0.25 mg. The calculator does this conversion for you.
Divide the target dose by the amount in each mL. First put both amounts in the same unit. For example, 250 mcg = 0.25 mg. At 2.5 mg/mL, 0.25 ÷ 2.5 = 0.1 mL of liquid. Your prescriber chooses the dose; this calculation finds the liquid volume.
On a U-100 syringe, 1 mL corresponds to 100 units, so 10 units = 0.1 mL and 1 unit = 0.01 mL. Check the syringe’s actual scale. U-40 and U-500 scales use different conversions and are not supported by this calculator.
No. U-100 syringe units measure liquid volume. Medication IU (International Units) measure how much a substance acts in the body. There is no single IU-to-mg conversion for every product. If your product uses IU, enter both the vial amount and target dose in IU. The result gives the liquid volume in mL.
It depends on concentration. On a U-100 syringe, 10 units = 0.1 mL. At 2.5 mg/mL that volume contains 0.25 mg; at 5 mg/mL it contains 0.5 mg. A unit marking alone cannot tell you the amount of medication.
Use the volume in your product’s instructions. There is no single amount that works for every product. Adding more liquid puts less peptide in each mL, so you need more liquid for the same dose. The comparison tool shows that math; it cannot decide which mixture is suitable.
Bacteriostatic water is sterile water with a preservative, such as benzyl alcohol. The preservative limits bacterial growth; it does not make contaminated liquid free of germs. Check the product’s instructions before choosing it.
Only if your product’s instructions allow it. Sterile water has no preservative; bacteriostatic water contains one; saline is salt water. They do not work the same way with every product. The calculator uses their volumes, but cannot tell you whether a substitute is suitable.
Yes. In Guided mode, choose More than one mixing liquid at the liquid step. In Advanced mode, find it under More options. You can enter up to three liquids, and the calculator adds their volumes. Only combine liquids if your product’s instructions allow it.
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No. mL measures space for liquid; mg measures the amount of peptide or medication. A 3 mL bottle can contain 5 mg, 10 mg, or another amount. Enter the vial size and peptide amount separately. The calculator warns you if the liquid volume is larger than the capacity you entered.
Not always. The powder and other ingredients can change the total volume after mixing. The calculator assumes the liquid volumes you enter equal that total. If your product gives a different final volume or concentration, use that information.
Follow the product’s written storage instructions and discard date. There is no rule that every mixed peptide lasts the same number of days. In particular, a preservative does not mean every mixture lasts 28 days. Ask your pharmacist if the instructions are unclear.
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Do not use it if you are unsure it is free of germs. Clear liquid can still be contaminated, even if it contains a preservative. Ask your pharmacist how to handle and discard it. Use a new sterile needle and syringe for each vial entry.
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The calculator works backward from your fill amount and target dose to look for simple concentrations and measurable draws. It rounds suggested mixing volumes to 0.01 mL, then recalculates the actual concentration. Selecting an injection option also shows a rounded draw and its estimated dose difference. Without a selected option, results stay exact apart from display rounding. Confirm both liquid measurement and dose differences with your pharmacist.
Do not assume the routes are interchangeable. Nasal formulation, pump performance, and absorption differ from injection. This calculator estimates concentration and the amount emitted by a full spray; it cannot determine an equivalent nasal dose, compatible ingredients, or how much is absorbed. Use product-specific instructions confirmed by your pharmacist or prescriber.
It means one full pump emits a nominal 0.1 mL, or 100 microliters (µL). As a math example, 5 mg/mL × 0.1 mL = 0.5 mg per spray; a 1 mg target would be two full sprays. This is not a dose recommendation. Check the actual device specification and priming instructions. A partial pump is not a reliable half-dose.
Choose the size of your actual syringe: 0.3, 0.5, 1, or 3 mL. The calculated liquid volume must fit within that capacity. Check the lines too: each line may mark a different amount on different syringes. Ask your pharmacist which device can measure your dose accurately.
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Rounding changes the dose. For example, 0.5 U-100 unit is 0.005 mL. Rounding up to 1 unit doubles the liquid and the dose. The calculator keeps the exact draw until you select a liquid suggestion with an explicitly rounded draw. The rounding details show the dose difference; the calculator also checks the illustrated markings. Ask your pharmacist how to measure the result accurately.
Divide the total amount by one dose, using the same units. For example, 5 mg ÷ 0.25 mg = 20 doses. Liquid left in the vial, needle, or syringe can mean fewer full doses. A decimal means part of another dose. Follow the storage and discard instructions.
The calculator multiplies the amount per mL by the liquid volume from one full pump. That gives the amount per spray. It then divides your target dose by that amount. Use your device’s stated volume and preparation instructions. A partial pump is not a reliable dose. Your prescriber must confirm whether nasal use is suitable.
Use Blend calculator mode to combine two to six single-compound vials, using your own amount of each compound per dose. It works for injections or a nasal spray bottle, accounts for powder or already mixed vials and the liquid left in them, and can mix in a new empty vial or inside one of your source vials kept whole. Guided and Advanced modes still describe one ingredient or a total mixture; a pre-made blend’s total weight does not identify its individual ingredient amounts. Blend calculations do not establish compatibility, solubility, sterility, stability or safe doses. Have a pharmacist verify the preparation before mixing.
